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dapi nuclear staining  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology dapi nuclear staining
    Dapi Nuclear Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+stain/DAPI/pm42869916-314-14-20
    Average 96 stars, based on 1459 article reviews
    dapi nuclear staining - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: The Reproductive Toxicity of Monosodium Glutamate by Damaging GnRH Neurons Cannot Be Relieved Spontaneously Over Time
    Article Snippet: Next, sections were rinsed with a 0.01 M PBS solution (pH 7.2) three times and incubated for 60 min at room temperature with a second antibody (DyLight 649 E32610, Abbkine Scientific Co., Ltd., CA, USA), goat anti-mouse IgG, diluted in blocking solution, with a dilution of 1:5000, after avoiding light. .. Next, DAPI stain (sc-24941, Santa Cruz Biotechnology, Inc., USA) was applied for three minutes. ..

    Article Title: The Reproductive Toxicity of Monosodium Glutamate by Damaging GnRH Neurons Cannot Be Relieved Spontaneously Over Time
    Article Snippet: Next, sections were rinsed with a 0.01 M PBS solution (pH 7.2) three times and incubated for 60 min at room temperature with a second antibody (DyLight 649 E32610, Abbkine Scientific Co., Ltd., CA, USA), goat anti-mouse IgG, diluted in blocking solution, with a dilution of 1:5000, after avoiding light. .. Next, DAPI stain (sc-24941, Santa Cruz Biotechnology, Inc., USA) was applied for three minutes. ..

    Article Title: Methods and compositions for treating neuroblastoma
    Article Snippet: .. After three 5 minutes washes, cells were incubated for 45 minutes in the dark with Rhodamine-conjugated goat anti-mouse secondary antibody (Jackson) then washed 3×5 minutes and coverslipped with mounting media containing DAPI stain (Santa Cruz) for evaluation with a fluorescence microscope. ..

    Article Title: Expression of Cell Membrane Antigens in Cells Excreted in the Urinary Sediment Predicts Progression of Renal Disease in Patients with Focal Segmental Glomerulosclerosis.
    Article Snippet: Background/Aims: A link between the number of podocytes excreted in the urine and activity of glomerular disease has been established.. The aim of this study was to investigate possible correlations between urinary cells’ phenotype and the progression of focal segmental glomerulosclerosis (FSGS).. Methods: Forty patients with newly diagnosed FSGS were included.

    Article Title: NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes
    Article Snippet: Antibody , DyLight 800 anti-mouse IgG (goat polyclonal) , Thermo Fisher Scientific , Cat# SA5-10176, RRID: AB_2556756 , Dilution 1:10000 (WB). .. Other , DAPI stain , SCBT , sc-3598 , (1.5 μg/ml). .. Other , Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane , Millipore Sigma , Cat# L2020 , 1 μg/μl.

    Incubation:

    Article Title: Methods and compositions for treating neuroblastoma
    Article Snippet: .. After three 5 minutes washes, cells were incubated for 45 minutes in the dark with Rhodamine-conjugated goat anti-mouse secondary antibody (Jackson) then washed 3×5 minutes and coverslipped with mounting media containing DAPI stain (Santa Cruz) for evaluation with a fluorescence microscope. ..

    Fluorescence:

    Article Title: Methods and compositions for treating neuroblastoma
    Article Snippet: .. After three 5 minutes washes, cells were incubated for 45 minutes in the dark with Rhodamine-conjugated goat anti-mouse secondary antibody (Jackson) then washed 3×5 minutes and coverslipped with mounting media containing DAPI stain (Santa Cruz) for evaluation with a fluorescence microscope. ..

    Microscopy:

    Article Title: Methods and compositions for treating neuroblastoma
    Article Snippet: .. After three 5 minutes washes, cells were incubated for 45 minutes in the dark with Rhodamine-conjugated goat anti-mouse secondary antibody (Jackson) then washed 3×5 minutes and coverslipped with mounting media containing DAPI stain (Santa Cruz) for evaluation with a fluorescence microscope. ..



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    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing